Chapitre D'ouvrage Année : 2017

Membrane Repair Assay for Human Skeletal Muscle Cells

Résumé

The characterization of the membrane repair machinery in human skeletal muscle has become crucial, since it has been shown that some muscular dystrophies result from a defect of this fundamental physiological process. Deciphering membrane repair mechanism requires the development of methodologies allowing studying the response of skeletal muscle cells to sarcolemma damage and identifying candidate proteins playing a role in the membrane repair machinery. Here, we describe a protocol that is based on the creation of cell membrane disruption by infrared laser irradiation in human myotubes. Membrane disruption and repair are assayed by monitoring the incorporation into myotubes of the membrane probe FM1-43. This methodology has recently enabled us to show that Annexin-A5 is required for membrane repair in human skeletal muscle cells (

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Dates et versions

hal-04884820 , version 1 (13-01-2025)

Identifiants

Citer

Romain Carmeille, Coralie Croissant, Flora Bouvet, Anthony Bouter. Membrane Repair Assay for Human Skeletal Muscle Cells. James G. Ryall. Skeletal Muscle Development, 1668, Springer, pp.195 - 207, 2017, Methods in Molecular Biology, 978-1-4939-7283-8. ⟨10.1007/978-1-4939-7283-8_14⟩. ⟨hal-04884820⟩
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