Poster De Conférence Année : 2024

Additional genotyping with breedwheat-410k-array evidenced new loci associated to nitrogen allocation to grain storage proteins

Résumé

The content and composition of glutenin and gliadin, the two major grain storage proteins (GSPs) of wheat, are the main determinants of the end-use value of bread wheat (Triticum aestivum L.) grain. We previously performed a genetic association study for allocation levels of grain nitrogen (N) to GSP classes/subunits by using an allometric scaling method with a worldwide core collection of 196 bread wheats cultivated in three environments. Besides structural GSP genes, several loci putatively trans-regulating GSPs such as transcriptional factors were detected as the candidate loci, although the number of markers used in the study was limited (Plessis et al. 2013).Here, we conducted a genome-wide association study (GWAS) on the previous phenotypic data sets with 250,129 SNPs additionally obtained by the BreedWheat 410K genotyping array, aiming to more comprehensive detection of relevant loci for quantity of GSP classes/subunits and grain N allocation. In the analysis of phenotypic data sets, Pearson correlation test and principal component analysis (PCA) on quantity of GSPs and grain N allocations revealed that glutenin and gliadin contents as well as the N partitioning among these proteins were not significantly correlated, suggesting that allocation factors are different between these major GSPs. On the other hand, grain N allocations to high- and low- molecular weight glutenin subunits demonstrated a significant negative correlation, indicating that some common molecular mechanism underlies the nitrogen partitioning between these subunits. In addition to a GWAS based on the allometric scaling parameters, a strategy of using PC-scores as dependent variables for the GWAS (PCA-based GWAS) on quantity of GSP classes/subunits and grain N allocation was applied expecting to extract key information from phenotypically these complex traits. The effectiveness of these methods in revealing the factors responsible for N allocation in wheat grain and the newly detected associated loci will be discussed.

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Dates et versions

hal-04883332 , version 1 (13-01-2025)

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  • HAL Id : hal-04883332 , version 1

Citer

Naoto Sano, Anne Plessis, Catherine Ravel, Pierre Martre, Jacques Le Gouis. Additional genotyping with breedwheat-410k-array evidenced new loci associated to nitrogen allocation to grain storage proteins. 3. International wheat congress, Sep 2024, Perth, Australia. ⟨hal-04883332⟩
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