Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress - Archive ouverte HAL
Article Dans Une Revue BMC Microbiology Année : 2018

Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress

Résumé

Background: Staphylococcus aureus is an important foodborne pathogen. Lactococcus garvieae is a lactic acid bacterium found in dairy products; some of its strains are able to inhibit S. aureus growth by producing H 2 O 2 . Three strains of L. garvieae from different origins were tested for their ability to inhibit S. aureus SA15 growth. Two conditions were tested, one in which H 2 O 2 was produced (high aeration) and another one in which it was not detected (low aeration). Several S. aureus genes related to stress, H 2 O 2 -response and virulence were examined in order to compare their level of expression depending on the inoculated L. garvieae strain. Simultaneous L. garvieae H 2 O 2 metabolism gene expression was followed.
Results:

The results showed that under high aeration condition, L. garvieae strains producing H 2 O 2 (N201 and CL-1183) inhibited S. aureus SA15 growth and impaired its ability to deal with hydrogen peroxide by repressing H 2 O 2 -degrading genes. L. garvieae strains induced overexpression of S. aureus stress-response genes while cell division genes and virulence genes were repressed. A catalase treatment partially or completely restored the SA15 growth. In addition, the H 2 O 2 non-producing L. garvieae strain (Lg2) did not cause any growth inhibition. The SA15 stress-response genes were down-regulated and cell division genes expression was not affected. Under low aeration condition, while none of the strains tested exhibited H 2 O 2 -production, the 3 L. garvieae strains inhibited S. aureus SA15 growth, but to a lesser extent than under high aeration condition.

Conclusion:

Taken together, these results suggest a L. garvieae strain-specific anti-staphylococcal mechanism and an H 2 O 2 involvement in at least two of the tested L. garvieae strains.

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Sciences du Vivant [q-bio]
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https://hal.science/hal-04840989

Soumis le : lundi 16 décembre 2024-15:29:28

Dernière modification le : vendredi 20 décembre 2024-11:01:21

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hal-04840989 , version 1 (16-12-2024)

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Clothilde Queiroux, Muriel Bonnet, Taous Saraoui, Pierre Delpech, Philippe Veisseire, et al.. Dialogue between Staphylococcus aureus SA15 and Lactococcus garvieae strains experiencing oxidative stress. BMC Microbiology, 2018, 18, ⟨10.1186/s12866-018-1340-3⟩. ⟨hal-04840989⟩

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