Subunit protein CD40.SARS.CoV2 vaccine induces SARS-CoV-2-specific stem cell-like memory CD8+ T cells - Archive ouverte HAL
Article Dans Une Revue EBioMedicine Année : 2025

Subunit protein CD40.SARS.CoV2 vaccine induces SARS-CoV-2-specific stem cell-like memory CD8+ T cells

Résumé

Background: Ideally, vaccination should induce protective long-lived humoral and cellular immunity. Current licensed COVID-19 mRNA vaccines focused on the spike (S) region induce neutralizing antibodies that rapidly wane. Methods: Herein, we show that a subunit vaccine (CD40.CoV2) targeting spike and nucleocapsid antigens to CD40-expressing cells elicits broad specific human (hu)Th1 CD4 + and CD8 + T cells in humanized mice. Findings: CD40.CoV2 vaccination selectively enriched long-lived spike-and nucleocapsid-specific CD8 + progenitors with stem-cell-like memory (Tscm) properties, whereas mRNA BNT162b2 induced effector memory CD8 + T cells. CD8 + Tscm cells produced IFNγ and TNF upon antigenic restimulation and showed a high proliferation rate. We demonstrate that CD40 activation is specifically required for the generation of huCD8 + Tscm cells. Interpretation: These results support the development of a CD40-vaccine platform capable of eliciting long-lasting T-cell immunity. Funding: This work was supported by Inserm, Université Paris-Est Créteil, and the Investissements d'Avenir program, Vaccine Research Institute (VRI), managed by the ANR.
Fichier principal
Vignette du fichier
1-s2.0-S2352396424005152-main.pdf (1.97 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Licence

Dates et versions

hal-04836386 , version 1 (13-12-2024)

Licence

Identifiants

Citer

Laury Nguema, Florence Picard, Marwa El Hajj, Léa Dupaty, Craig Fenwick, et al.. Subunit protein CD40.SARS.CoV2 vaccine induces SARS-CoV-2-specific stem cell-like memory CD8+ T cells. EBioMedicine, 2025, 111, pp.105479. ⟨10.1016/j.ebiom.2024.105479⟩. ⟨hal-04836386⟩

Collections

INSERM IMRB UPEC
0 Consultations
0 Téléchargements

Altmetric

Partager

More