Selective autophagy impedes KSHV entry after recruiting the membrane damage sensor galectin-8 to virus-containing endosomes. - Archive ouverte HAL
Article Dans Une Revue Cell Reports Année : 2024

Selective autophagy impedes KSHV entry after recruiting the membrane damage sensor galectin-8 to virus-containing endosomes.

Katarina Wendy Schmidt
  • Fonction : Auteur
Charlotte Montespan
  • Fonction : Auteur
Danielle Thompson
  • Fonction : Auteur
Miriam S Lucas
  • Fonction : Auteur
Laure-Anne Ligeon
  • Fonction : Auteur
Alexander S Hahn
  • Fonction : Auteur
Urs F Greber
  • Fonction : Auteur
Christian Münz
  • Fonction : Auteur

Résumé

Kaposi sarcoma-associated herpesvirus (KSHV) is an oncogenic γ-herpesvirus. Autophagy during KSHV entry has remained unexplored. We show that LC3 lipidation as a hallmark of autophagy is induced shortly after KSHV entry. LC3 co-localizes with KSHV in amphisomes during entry and loss of LC3 lipidation increases infection. Accordingly, NDP52, a receptor of selective autophagy, was recruited to endocytosed viral particles, and its reduction increased KSHV infection. Additionally, virus particles co-localized with the endolysosome damage sensor galectin-8 upon KSHV entry and depletion of galectin-8 promoted KSHV infection. Compared with herpes simplex virus, listeriolysin, adenovirus, and influenza virus, and in contrast to what was previously thought about enveloped viruses, KSHV binding to EphA2 by its envelope protein gH causes endolysosomal membrane damage, akin to non-enveloped viruses and bacteria. Taken together, our study identifies an important anti-viral role for galectin-8, NDP52, and the autophagy machinery at virus-damaged endosomes, restricting KSHV entry by selective autophagy.
Fichier non déposé

Dates et versions

hal-04818206 , version 1 (04-12-2024)

Identifiants

Citer

Katarina Wendy Schmidt, Charlotte Montespan, Danielle Thompson, Miriam S Lucas, Laure-Anne Ligeon, et al.. Selective autophagy impedes KSHV entry after recruiting the membrane damage sensor galectin-8 to virus-containing endosomes.. Cell Reports, 2024, 43 (12), pp.115019. ⟨10.1016/j.celrep.2024.115019⟩. ⟨hal-04818206⟩

Collections

CNRS MFP
0 Consultations
0 Téléchargements

Altmetric

Partager

More