Optical Manipulation and Recording of Neural Activity with Wavefront Engineering
Résumé
The first section of this chapter is dedicated to a brief overview of some basic principles of neural physiology relevant for controlling and recording neuronal activity using light. Then, the selection of appropriate actuators and sensors for manipulating and monitoring particular neural signals is discussed, with particular attention paid to kinetics and sensitivity. Some considerations for minimizing crosstalk in optical neurophysiology experiments are also introduced. Next, an overview of the state-of-the-art optical technologies is provided, including a description of suitable laser sources for two-photon excitation according to particular experimental requirements. Finally, some detailed, technical, information regarding the specific wavefront engineering approaches known as Generalized Phase Contrast (GPC) and temporal focusing is provided.
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