This work assessed the influence of wastewater (WW) composition and bioaggregates type on the content, chemical and gelling properties of alginate-like exopolymers (ALE). Results indicate that the WW composition/aggregate type have limited influence on the ALE content. ALE was mainly composed of proteins, humics acids and uronic acids. Only the ALE extracted from granules fed with acetate/propionate featured mannuronate content. ALE extracted from aggregate fed with real WW had high humic acids content. All extracts were suitable to form gels, what is attributed to the presence of uronic acids. The rheological properties of the gels were directly influenced by the composition of the ALE extract: ALE from granules fed with soluble COD had the highest and from activated sludge fed with real WW the lowest storage modulus G'. Linking growth conditions to the gel properties is relevant for full-scale recovery of ALE and its industrial application.