Macaque species, specifically Macaca mulatta and M. fascicularis, play a pivotal role in neuroscience and biomedical research. Inadequate housing conditions and husbandry practices can lead to physiological and behavioral alterations, reflecting poor welfare, and can have an impact on the validity of results. However, despite the existence in the scientific literature of numerous studies examining welfare indicators for laboratory- housed macaques, few field studies have been carried to develop a species-specific welfare assessment tool for macaques and test its validity. This is the goal of this project. This study was conducted on 169 macaques housed in eight research laboratories in France. We aimed to investigate their welfare through a welfare assessment tool. This latter is based on pre-existing frameworks on the matter, such as AWIN (Animal Welfare INdicators) protocol for farm animals, but specifically adapted to macaques in laboratory settings. Behavioral data were collected by two observers on each subject with 10-min of focal sampling, totaling 110 hours of observations. The interrater reliability score was calculated using intraclass correlation coefficient (ICC). In addition, a wide range of animal-based and environmental-based measures were collected through a survey which includes over a hundred questions divided into 4 categories: housing, nutrition, health, and behavior. The ongoing data analysis, including Generalized Linear Mixed Models, investigate the potential impact of various housing conditions and husbandry practices on the behaviors exhibited by macaques. Preliminary results suggest that single housing conditions and water restriction influence the proportions of abnormal behaviors exhibited by macaques, in line with previous studies reported for other species. Through this work, our aim is to guarantee an objective and standardized assessment of the welfare of primates in the laboratory, to make recommendations for improving husbandry practices and to help raise awareness of the welfare of laboratory animals, for ethical and responsible research.