Carbon-14 (14C) has a natural origin but is also anthropogenically released from civil nuclear facilities. Due to its long decay period (half-life: 5700 ± 30 years), it is a persistent radionuclide in the environment. In rivers, the complex speciation of carbon makes the fate of industrial 14C difficult to track. This study reports a first overview of artificial 14C cycling in a nuclearized river. A one-year sampling campaign was conducted on the French nuclearized Rhône River and two of its non-nuclearized tributaries (Durance and Ardèche rivers). Isotopic (δ13C, Δ14C) and carbon concentrations analyses were performed on the particulate organic carbon (POC), dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC). Chlorophyll-a (Chl-a) and tritium analyses were performed to assess the dynamic of aquatic organic matter and the nuclear industry contribution, respectively. Comparisons of Δ14C data obtained from the Rhône River with those from the tributaries highlight significant industrial radiocarbon labelling in all carbon forms, with medians of 142, 130 and 42 ‰ for POC, DOC and DIC, that are 2–3 times higher than those of the tributaries. The high values of Chl-a/POC ratios with Δ14C-enriched POC suggest a biological uptake of artificial Δ14C in DIC by aquatic photosynthesis. The relationship of Δ14C-DIC with tritium activity indicates a response to recent releases and enables the contribution of nuclear power plants to be estimated at a median of 26 %. Sampling at the Rhône's mouth would reinforce our understanding of the fate of riverine 14C when entering the marine environment.