The long non-coding RNA lncRNA-DRNR enhances infectious bronchitis virus replication by targeting chicken JMJD6 and modulating interferon-stimulated genes expression via the JAK-STAT signalling pathway - Archive ouverte HAL
Article Dans Une Revue Veterinary Research Année : 2024

The long non-coding RNA lncRNA-DRNR enhances infectious bronchitis virus replication by targeting chicken JMJD6 and modulating interferon-stimulated genes expression via the JAK-STAT signalling pathway

Wenjun Yan
  • Fonction : Auteur
Xue Fu
  • Fonction : Auteur
Hao Li
  • Fonction : Auteur
Kailu Wang
  • Fonction : Auteur
Cailiang Song
  • Fonction : Auteur
Chengyao Hou
  • Fonction : Auteur
Cangwei Lei
  • Fonction : Auteur
Hongning Wang
  • Fonction : Auteur
Xin Yang
Connectez-vous pour contacter l'auteur

Résumé

AbstractInfectious bronchitis virus (IBV) is the causative agent of infectious bronchitis (IB), a severe disease that primarily affects young chickens and poses a significant challenge to the global poultry industry. Understanding the complex interaction between the virus and its host is vital for developing innovative antiviral strategies. Long non-coding RNA (lncRNA) plays a crucial role in regulating host antiviral immune responses. Our previous studies have shown that IBV infection disrupts the stability of lncRNA in host cells, indicating a potential regulatory role for lncRNA in IBV pathogenesis. It is still not clear how lncRNA precisely modulates IBV replication. In this study, we observed down-regulation ofMSTRG.26120.58 (named lncRNA-DRNR) expression in various chicken cell lines upon IBV infection. We demonstrated that silencing lncRNA-DRNR using siRNA enhances intracellular replication of IBV. Through exploring genes encoding proteins upstream and downstream of lncRNA-DRNR within a 100 kb range, we identified chJMJD6 (chicken JMJD6) as a potential target gene negatively regulated by lncRNA-DRNR expression levels. Furthermore, chJMJD6 inhibits STAT1 methylation, thereby affecting the induction of interferon-stimulated genes (ISGs) through the activation of the IFN-β-mediated JAK-STAT signalling pathway, ultimately promoting the intracellular replication of IBV. In summary, our findings reveal the critical role played by lncRNA-DRNR during IBV infection, providing novel insights into mechanisms underlying coronavirus-induced disruption in lncRNA stability.
Fichier principal
Vignette du fichier
13567_2024_Article_1396.pdf (3.24 Mo) Télécharger le fichier
Origine Publication financée par une institution

Dates et versions

hal-04776076 , version 1 (11-11-2024)

Identifiants

Citer

Wenjun Yan, Xue Fu, Hao Li, Kailu Wang, Cailiang Song, et al.. The long non-coding RNA lncRNA-DRNR enhances infectious bronchitis virus replication by targeting chicken JMJD6 and modulating interferon-stimulated genes expression via the JAK-STAT signalling pathway. Veterinary Research, 2024, 55 (1), pp.141. ⟨10.1186/s13567-024-01396-6⟩. ⟨hal-04776076⟩

Collections

ARINRAE-VR ARINRAE
6 Consultations
2 Téléchargements

Altmetric

Partager

More