Anaplasma phagocytophilum (Aph) is a strict intracellular bacterium transmitted by ticks of the genus Ixodes. It causes granulocytic anaplasmosis (GA) in humans and various species of domestic mammals (dogs, cattle, sheep...) also called tick-borne fever (TBF) in ruminants. Contrary to TBF which is particularly prevalent in Europe and is the cause of significant economic losses, human GA is mainly found in the USA. Aph is characterized by high genetic diversity and complex epidemiological cycles, as it is also found in many wild animals (deer, rodents, birds...). Domestic species such as horses and dogs, as well as wild species such as wild boar, foxes and hedgehogs, harbor strains that are suspected of being zoonotic. Conversely, deer, rodents (voles) and shrews, as well as birds apparently, appear to harbor strains that are genetically distant from those that affect humans. Birds play an important role in the maintenance and spread of pathogens. Aph DNA has been identified in avian hosts in Europe, with prevalence rates ranging from 0% to 33.8%. However, few epidemiologic studies have been conducted on birds and Aph. Our project therefore aimed to investigating the presence of Aph in birds from urban and peri-urban areas of Ile-de-France and neighboring departments, particularly in passerines. Liver, spleen and skin samples from 680 passerines were tested for the presence of Aph DNA by qPCR targeting the msp2 gene. Three blackbirds (Turdus merula) were tested positive for Aph DNA. This corresponds to a detection rate of 0.4 % in all the birds tested and 3.3 % in the blackbirds. In a second phase, a phylogenetic study was performed targeting the groEL and ankA genes and using MLST (multilocus sequence typing). These results support the existence of an epidemiological cycle specific to birds, in France, in agreement with what is found in other European countries.