Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation - Archive ouverte HAL
Article Dans Une Revue STAR Protocols Année : 2024

Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation

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Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation

The development of fast ligation chemistries for the site-specific modification of proteins has become a major focus in chemical biology. We describe steps for preparing an oxalyl thioester precursor in the form of an N-oxalyl perhydro-1,2,5-dithiazepine handle, i.e., the oxo SEA group, and incorporating it into a peptide modifier using solid phase peptide synthesis. We then detail procedures for its application for the modification of an N-terminal Cys-containing B1 domain of the streptococcal G protein using the native chemical ligation.

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hal-04751542 , version 1 (24-10-2024)

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Francesco Terzani, Chen Wang, Simindokht Rostami, Rémi Desmet, Benoît Snella, et al.. Protocol for protein modification using oxalyl thioester-mediated chemoselective ligation. STAR Protocols, 2024, 5 (4), pp.103390. ⟨10.1016/j.xpro.2024.103390⟩. ⟨hal-04751542⟩
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