New microsystem for gradual electroporation of a regular spheroid population and application for protocol comparison
Résumé
A microfluidic platform specifically designed by our team for culture, electroporation, and observation of spheroids with regular size and shape has been described in a previous communication.
We present in this study a microfluidic device derived from this work designed to apply a gradient of electric field to a spheroid population, in order to investigate effect of electroporation (EPN) parameters on a spheroid model. Fluorescence microscopy is used to estimate the efficiency of EPN, it allows for the direct plot of permeabilization-intensity relationship in a single experiment.