Plant cell wall enzymatic deconstruction: Bridging the gap between micro and nano scales - Archive ouverte HAL
Article Dans Une Revue Bioresource Technology Année : 2024

Plant cell wall enzymatic deconstruction: Bridging the gap between micro and nano scales

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Understanding lignocellulosic biomass resistance to enzymatic deconstruction is crucial for its sustainable conversion into bioproducts. Despite scientific advances, quantitative morphological analysis of plant deconstruction at cell and tissue scales remains under-explored. In this study, an original pipeline is devised, involving fourdimensional (space + time) fluorescence confocal imaging, and a novel computational tool, to track and quantify deconstruction at cell and tissue scales. By applying this pipeline to poplar wood, dynamics of cellular parameters was computed and cellulose conversion during enzymatic deconstruction was measured. Results showed that enzymatic deconstruction predominantly impacts cell wall volume rather than surface area. Additionally, a negative correlation was observed between pre-hydrolysis compactness measures and volumetric cell wall deconstruction rate, whose strength was modulated by enzymatic activity. Results also revealed a strong positive correlation between average volumetric cell wall deconstruction rate and cellulose conversion rate. These findings link key deconstruction parameters across nano and micro scales.
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hal-04735225 , version 1 (14-10-2024)

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Yassin Refahi, Aya Zoghlami, Thibaut Viné, Christine Terryn, Gabriel Paës. Plant cell wall enzymatic deconstruction: Bridging the gap between micro and nano scales. Bioresource Technology, 2024, 414, pp.131551. ⟨10.1016/j.biortech.2024.131551⟩. ⟨hal-04735225⟩
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