Equine coronavirus infection and replication in equine intestinal enteroids - Archive ouverte HAL
Article Dans Une Revue Veterinary Research Année : 2024

Equine coronavirus infection and replication in equine intestinal enteroids

Yoshinori Kambayashi
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Manabu Nemoto
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Akihiro Ochi
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Daiki Kishi
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Takanori Ueno
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Koji Tsujimura
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Hiroshi Bannai
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Nanako Kawanishi
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Minoru Ohta
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Tohru Suzuki
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Résumé

AbstractIn this study, equine intestinal enteroids (EIEs) were generated from the duodenum, jejunum, and ileum and inoculated with equine coronavirus (ECoV) to investigate their suitability as in vitro models with which to study ECoV infection. Immunohistochemistry revealed that the EIEs were composed of various cell types expressed in vivo in the intestinal epithelium. Quantitative reverse-transcription PCR (qRT-PCR) and virus titration showed that ECoV had infected and replicated in the EIEs. These results were corroborated by electron microscopy. This study suggests that EIEs can be novel in vitro tools for studying the interaction between equine intestinal epithelium and ECoV.
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Dates et versions

hal-04734359 , version 1 (14-10-2024)

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Yoshinori Kambayashi, Manabu Nemoto, Akihiro Ochi, Daiki Kishi, Takanori Ueno, et al.. Equine coronavirus infection and replication in equine intestinal enteroids. Veterinary Research, 2024, 55 (1), pp.135. ⟨10.1186/s13567-024-01381-z⟩. ⟨hal-04734359⟩

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