A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains - Archive ouverte HAL
Article Dans Une Revue Veterinary Research Année : 2024

A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains

Yang Li
  • Fonction : Auteur
Yumiao Wang
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Xiuxiu Pei
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Shao Chen
  • Fonction : Auteur
Yang Jing
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Yongshuai Wu
  • Fonction : Auteur
Zhiqian Ma
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Zhiwei Li
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Zifang Zheng
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Yingtong Feng
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Lele Xu
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Xiao Liu
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Xuyang Guo
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Haixue Zheng
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Shuqi Xiao
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Résumé

AbstractPorcine reproductive and respiratory syndrome (PRRS) is one of the most significant swine viral infectious diseases worldwide. Vaccination is a key strategy for the control and prevention of PRRS. At present, the NADC30-like PRRSV strain has become the predominant epidemic strain in China, superseding the HP-PRRSV strain. The existing commercial vaccines offer substantial protection against HP-PRRSV, but their efficacy against NADC30-like PRRSV is limited. The development of a novel vaccine that can provide valuable cross-protection against both NADC30-like PRRSV and HP-PRRSV is highly important. In this study, an infectious clone of a commercial MLV vaccine strain, GD (HP-PRRSV), was first generated (named rGD). A recombinant chimeric PRRSV strain, rGD-SX-5U2, was subsequently constructed by using rGD as a backbone and embedding several dominant immune genes, including the NSP2, ORF5, ORF6, and ORF7 genes, from an NADC30-like PRRSV isolate. In vitro experiments demonstrated that chimeric PRRSV rGD-SX-5U2 exhibited high tropism for MARC-145 cells, which is of paramount importance in the production of PRRSV vaccines. Moreover, subsequent in vivo inoculation and challenge experiments demonstrated that rGD-SX-5U2 confers cross-protection against both HP-PRRSV and NADC30-like PRRSV, including an improvement in ADG levels and a reduction in viremia and lung tissue lesions. In conclusion, our research demonstrated that the chimeric PRRSV strain rGD-SX-5U2 is a novel approach that can provide broad-spectrum protection against both HP-PRRSV and NADC30-like PRRSV. This may be a significant improvement over previous MLV vaccinations.
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Dates et versions

hal-04734356 , version 1 (14-10-2024)

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Yang Li, Yumiao Wang, Xiuxiu Pei, Shao Chen, Yang Jing, et al.. A chimeric strain of porcine reproductive and respiratory syndrome virus 2 derived from HP-PRRSV and NADC30-like PRRSV confers cross-protection against both strains. Veterinary Research, 2024, 55 (1), pp.132. ⟨10.1186/s13567-024-01390-y⟩. ⟨hal-04734356⟩
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