Marine fish are often speckled with “black spots” caused by host response to larval trematode infection. These unattractive spots and the presence of parasites may lead to important economic loss in fishery and may have impacts on public health. As many other zoonotic trematodes, Cryptocotyle (Lühe, 1899) are present in marine fish species. Human may be infected by consumption of raw or undercooked fish products. Characterized as zoonotic by Chai and Jung (2017) based on Babbott et al. (1961) human case description in 1999, its impact on human health is still unclear. For the first time, this study intends to characterize Cryptocotyle zoonotic potential. Beforehand conditions of conservation of Cryptocotyle metacercariae were set to organize scheduling of isolation of metacercariae from fish. Then, in vivo trials were performed on mice. After ingestion of 500 Cryptocotyle ligua metacercariae per mouse, daily monitoring of mice was undertaken by observation of their behavior, weight record and fecal time report. Mice were sacrificed twenty-one days post infection and organs were collected for observations of parasites or lesions. This survey constitutes the first analysis of Cryptocotyle lingua zoonotic potential by in vivo experiments. References: Babbott, F. L. J. , Frye, W. W., & Gordon, J. E. (1961). Intestinal parasites of man in Artic Greenland. The American journal of tropical medicine and hygiene, 10, 185-190. Chai, J. Y., & Jung, B.-K. (2017). Fishborne zoonotic heterophyid infections: An update [Article]. Food and Waterborne Parasitology, 8-9, 33-63.