Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from off-the-shelf antibodies
Résumé
Protocol to generate, purify, and analyze antibody-oligonucleotide conjugates from offthe-shelf antibodies Antibody-oligonucleotide conjugates (AOCs) are a fast-expanding modality for targeted delivery of therapeutic oligonucleotides to tissues. Here, we present a protocol to generate, purify, and analyze AOCs from off-the-shelf antibodies. We describe steps to conjugate single/doublestranded oligonucleotides bearing amine handles to linkers and, then, to antibodies using wellestablished chemistry. In addition, we provide details regarding the purification techniques and analytical methods suitable for AOC. This protocol can be applied for several purposes where AOC is a modality of interest.
Domaines
Chimie| Origine | Fichiers produits par l'(les) auteur(s) |
|---|