LCxSFC valve technologies: guidelines towards a successful on-line modulation - Archive ouverte HAL
Article Dans Une Revue Analytical Chemistry Année : 2024

LCxSFC valve technologies: guidelines towards a successful on-line modulation

Résumé

On-line two-dimensional liquid chromatography can be used under various separation modes but are sometimes limited in terms of orthogonality, especially for the analysis of neutral compounds. The use of SFC in the second dimension offers a wide choice of mobile and stationary phases, suggesting retention properties complementary to those of the first dimension. Initial works on on-line LCxSFC coupling published in the literature highlighted the first difficulties of solvent compatibility or potential problems about bubbles created by CO2 in loops. This present work highlights the impact of the interface between the LC and SFC dimensions on the performances of the 2D separation. Six different configurations have been evaluated, differing by an addition of a make-up flow in the first dimension, a split, or a separation of flow paths in the second dimension. Their performances with empty loops eliminated the need of complex trapping columns, whatever the LC mobile phase nature. Injection in partial fill mode was possible without any problems of CO2 bubbles formation, solvent miscibility, pressure, or modulation repeatability in each of the studied configurations. Selected configurations even allowed the use of a mobile phase split upstream of the valve, so that the on-line LCxSFC configuration could be as flexible as LCxLC instrumentation in term of first dimension flow rate or second dimension injected volume. Obtained results allowed building guidelines presenting the best interfaces to set up depending on the nature of the mobile phases in both dimensions. Two applications on monomers of lignin and industrial polymers confirmed preliminary results on configuration testing and illustrated that on-line LCxSFC could now be easily implemented. Precision tests on retention time were conclusive and are promising for the future use of this technique in industrial routines.
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Dates et versions

hal-04727276 , version 1 (09-10-2024)

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Margaux Sanchez, Florent Rouvière, Julien Crepier, Karine Faure. LCxSFC valve technologies: guidelines towards a successful on-line modulation. Analytical Chemistry, 2024, 276 (39), pp.126295. ⟨10.1021/acs.analchem.4c03263⟩. ⟨hal-04727276⟩
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