Chromatography membranes are used on an industrial scale for biotechnology applications and developments for food applications are described for milk and egg proteins [1]. Chromatographic purification of food proteins can improve functional properties but is not economically viable. Therefore, chromatography membranes present an interesting alternative as their high surface area and low flow resistance allow rapid separation processes, making them ideal for handling large volumes of feed quickly. In this study, the feasibility of replacing resin chromatography with membrane techniques for the purification of plant globulins from oleaginous and pulse proteins sources was evaluated. The fractionation of proteins extracted from pea seeds and rapeseed on chromatography resins (Hitrap FF Q and SP Cytiva, 1mL) was compared with membrane fractionation (Mustang Q and S Acrodisc Pall, 0.86mL). Firstly, separation conditions were validated on a syringe filter-sized membrane and binding capacity was assessed to optimize sample loading. For pea, anion exchangers separated 7S and 11S globulins and for rapeseed, cation exchangers retained 2S albumins while 12S globulins were eluted in the flow-through. Upscaling was then evaluated by multiplying cycles to produce a few grams of proteins using the Sartobind 20mL membrane (Sartorius) in step elution. Membrane separation was achieved in 4 and 6 min, whereas resins required 30 and 50 min for pea and rapeseed, respectively. Compared to resins, the membrane binding capacity of the cation exchanger was lower (4mg protein/mL membrane) while that of the anion exchanger was equivalent (45mg protein/mL membrane). The developed membrane-based procedure was easily up-scaled and led to efficient fractionation of plant proteins in a shorter time compared to resin chromatography, thus increasing productivity. This may open routes to produce food ingredients at industrial scale. [1] Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras & Sandra E. Kentish (30 Jun 2023): The Transition from Resin Chromatography to Membrane Adsorbers for Protein Separations at Industrial Scale, Separation & Purification Reviews, DOI: 10.1080/15422119.2023.2226128