Time-resolved crystallography captures light-driven DNA repair - Archive ouverte HAL
Article Dans Une Revue Science Année : 2023

Time-resolved crystallography captures light-driven DNA repair

Nina-Eleni Christou
Center for Free-Electron Laser Science
Virginia Apostolopoulou
Center for Free-Electron Laser Science
Diogo Melo
  • Fonction : Auteur
European XFEL Gmbh
Matthias Ruppert
Universität Hamburg
Alisia Fadini
Imperial College London
Alessandra Henkel
Center for Free-Electron Laser Science
Janina Sprenger
Center for Free-Electron Laser Science
Dominik Oberthuer
Center for Free-Electron Laser Science
Sebastian Günther
Center for Free-Electron Laser Science
Anastasios Pateras
Center for Free-Electron Laser Science
Aida Rahmani Mashhour
Center for Free-Electron Laser Science
Oleksandr Yefanov
Center for Free-Electron Laser Science
Marina Galchenkova
Center for Free-Electron Laser Science
Patrick Reinke
Center for Free-Electron Laser Science
Viviane Kremling
Center for Free-Electron Laser Science
T. Emilie S. Scheer
Center for Free-Electron Laser Science
Esther Lange
Center for Free-Electron Laser Science
Philipp Middendorf
Center for Free-Electron Laser Science
Robin Schubert
European XFEL Gmbh
Elke de Zitter
Institut de biologie structurale
CV
Koya Lumbao-Conradson
SLAC National Accelerator Laboratory
Jonathan Herrmann
  • Fonction : Auteur
Stanford University
Simin Rahighi
  • Fonction : Auteur
Stanford University
Ajda Kunavar
Stanford University
Emma Beale
Paul Scherrer Institute
John Beale
Paul Scherrer Institute
Claudio Cirelli
Paul Scherrer Institute
Philip Johnson
Paul Scherrer Institute
Florian Dworkowski
Paul Scherrer Institute
Dmitry Ozerov
Paul Scherrer Institute
Quentin Bertrand
Paul Scherrer Institute
Maximilian Wranik
Paul Scherrer Institute
Camila Bacellar
Paul Scherrer Institute
Saša Bajt
Center for Free-Electron Laser Science
Soichi Wakatsuki
Stanford University
Jonas Sellberg
Stanford University
Nils Huse
Universität Hamburg
Dušan Turk
Universität Hamburg
Henry Chapman
Center for Free-Electron Laser Science
Thomas Lane
Center for Free-Electron Laser Science

Résumé

Photolyase is an enzyme that uses light to catalyze DNA repair. To capture the reaction intermediates involved in the enzyme’s catalytic cycle, we conducted a time-resolved crystallography experiment. We found that photolyase traps the excited state of the active cofactor, flavin adenine dinucleotide (FAD), in a highly bent geometry. This excited state performs electron transfer to damaged DNA, inducing repair. We show that the repair reaction, which involves the lysis of two covalent bonds, occurs through a single-bond intermediate. The transformation of the substrate into product crowds the active site and disrupts hydrogen bonds with the enzyme, resulting in stepwise product release, with the 3′ thymine ejected first, followed by the 5′ base.

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https://hal.science/hal-04717786

Soumis le : vendredi 18 octobre 2024-11:50:12

Dernière modification le : vendredi 18 octobre 2024-15:23:03

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hal-04717786 , version 1 (18-10-2024)

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Nina-Eleni Christou, Virginia Apostolopoulou, Diogo Melo, Matthias Ruppert, Alisia Fadini, et al.. Time-resolved crystallography captures light-driven DNA repair. Science, 2023, 382 (6674), pp.1015-1020. ⟨10.1126/science.adj4270⟩. ⟨hal-04717786⟩

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