Novel freeform optical design methods can be classified in two categories, depending on whether they focus on the generation of a starting point or the development of new optimization tools. In this paper, we design a freeform three-mirror anastigmat (TMA) and compare different surface representations using either a differential ray tracer as a new optimization tool or a commercial ray tracer (ANSYS-ZEMAX OpticStudio). For differential ray tracing, we used FORMIDABLE (Freeform Optics Raytracer with Manufacturable Imaging Design cApaBiLitiEs), an optical design library with differential ray tracing and Non-Uniform Rational B-Splines (NURBS) optimization capabilities, available under the European Software Community License (ESCL). NURBS allow a freeform surface to be represented without needing any prior knowledge of the surface, such as the polynomial degree in polynomial descriptions. OpticStudio and other commercial optical design software are designed to optimize polynomial surfaces but are not well-suited to optimize NURBS surfaces, requiring a custom optical design library. In order to demonstrate the interest in using NURBS representation, we designed and independently optimized two freeform telescopes over different iteration cycles; with NURBS using FORMIDABLE or with XY polynomials using OpticStudio. We then compared the resulting systems using their root mean square field maps to assess the optimization quality of each surface representation. We also provided a full-system comparison, including mirror freeform departures. This study shows that NURBS can be a relevant alternative to XY polynomials for the freeform optimization of reflective three-mirror telescopes as it achieves more a uniform imaging quality in the field of view.