Searching for interactions at the membrane interface that confer antagonistic activities on floral regulators FT and TFL1
Résumé
The switch from leaf to flower producing meristems is controlled by FLOWERING LOCUS T ( which is the major component of the "florigen", a systemic signal that moves from leaves to shoot meristems and triggers the floral transition. In Arabidopsis its effect on flowering is repressed by TFL1 which is expressed in the shoot meristems. Despite their opposite functions, FT and TFL1 are members of the same family, namely PHOSPHATIDYLETHANOLAMINE BINDING PROTEINs. Although their binding to phosphatidylethanolamines are still unclear, they were shown to bind phosphatidylcholines that are major phospholipids of plant membranes and whose concentration impacts flowering time (Nakamura et al., 2014). In this context, we are investigating the ability of Arabidopsis FT and TFL1 to interact with membrane proteins and lipids by biochemical and biophysical approaches.
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