Article Dans Une Revue Methods in Molecular Biology Année : 2025

Simultaneous profiling of transcripts and genomic regions associated with nuclear bodies using the RNA-DNA High-salt Recovered Sequence (RD-HRS) method

Résumé

Regulation of many nuclear functions in eukaryotic cells, such as transcription and splicing, relies on the association of specific genomic loci and/or transcripts with nuclear microenvironments having high molecular crowding, such as nuclear bodies. These membrane-less organelles are typically self-assembled through mechanisms of phase separation and, because of their liquid-like nature, analyzing their composition remains challenging. Here we present a detailed protocol of the RNA-DNA High-salt Recovered Sequence (RD-HRS) method, an extended version of the High-salt Recovered Sequencesequencing (HRS-seq). Using high-salt treatments to make nuclear bodies insoluble, this extended version allows the profiling of both their RNA and genomic DNA contents, simultaneously, from the same biological sample.

Fichier principal
Vignette du fichier
Lecouvreur-et-al_RD-HRS_HAL.pdf (438.84 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

hal-04711849 , version 1 (27-09-2024)

Licence

Identifiants

Citer

Nathan Lecouvreur, Cosette Rebouissou, Annick Lesne, Thierry Forné. Simultaneous profiling of transcripts and genomic regions associated with nuclear bodies using the RNA-DNA High-salt Recovered Sequence (RD-HRS) method. Methods in Molecular Biology, 2025, Methods in Molecular Biology, 2962, pp.141-151. ⟨10.1007/978-1-0716-4726-4_10⟩. ⟨hal-04711849⟩
431 Consultations
242 Téléchargements

Altmetric

Partager

  • More