Biogenesis of cytochrome b$_6$ in photosynthetic membranes - Archive ouverte HAL
Article Dans Une Revue Journal of Cell Biology Année : 2009

Biogenesis of cytochrome b$_6$ in photosynthetic membranes

Résumé

In chloroplasts, binding of a c′-heme to cytochrome b$_6$ on the stromal side of the thylakoid membranes requires a specific mechanism distinct from the one at work for c-heme binding to cytochromes f and c$_6$ on the lumenal side of membranes. Here, we show that the major protein components of this pathway, the CCBs, are bona fide transmembrane proteins. We demonstrate their association in a series of hetero-oligomeric complexes, some of which interact transiently with cytochrome b$_6$ in the process of heme delivery to the apoprotein. In addition, we provide preliminary evidence for functional assembly of cytochrome b6f complexes even in the absence of c′-heme binding to cytochrome b$_6$. Finally, we present a sequential model for apo- to holo-cytochrome b$_6$ maturation integrated within the assembly pathway of b$_6$f complexes in the thylakoid membranes.
Fichier principal
Vignette du fichier
jcb_200812025.pdf (5.01 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-04704432 , version 1 (20-09-2024)

Licence

Identifiants

Citer

Denis Saint-Marcoux, Francis-André Wollman, Catherine de Vitry. Biogenesis of cytochrome b$_6$ in photosynthetic membranes. Journal of Cell Biology, 2009, 185 (7), pp.1195-1207. ⟨10.1083/jcb.200812025⟩. ⟨hal-04704432⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

More