Arsenic release from aquifers can be a major issue for aquifer storage and recovery (ASR) schemes and understanding the processes that release and attenuate As during ASR is the first step towards managing this issue. This study utilised the first and fourth cycles of a full scale field trial to examine the fate of As within the injectant plume during all stages of the ASR cycle, and the resultant water quality. The average recovered As concentration was greater than the source concentration; by 0.19 μmol/L (14 μg As/L) in cycle 1 and by 0.34 μmol/L (25 μg As/L) in cycle 4, indicating that As was being released from the aquifer sediments during ASR and the extent of As mobilisation did not decline with subsequent cycles. In the injection phase, As mobilisation due to oxidation of reduced minerals was limited to an oxic zone in close proximity to the ASR well, while desorption from Fe oxyhydroxide or oxide surfaces by injected P occurred further in the near well zone (0–4 m from the ASR well). With further aquifer passage during injection and greater availability of sorption sites there was evidence of attenuation via adsorption to Fe oxyhydroxides which reduced concentrations on the outer fringes of the injectant plume. During the period of aquifer storage, microbial activity resulting from the injection of organic matter resulted in increased As mobility due to reductive Fe oxyhydroxide dissolution and the subsequent loss of sorption sites and partial reduction of As(V) to the more mobile As(III). A reduced zone directly around the ASR well produced the greatest As concentration and illustrated the importance of Fe oxyhydroxides for controlling As concentrations. Given the small spatial extent of this zone, this process had little effect on the overall recovered water quality.