Enzymatically-derived oligo-carrageenans interact with α-Gal antibodies and Galectin-3 - Archive ouverte HAL
Journal Articles Carbohydrate Polymers Year : 2024

Enzymatically-derived oligo-carrageenans interact with α-Gal antibodies and Galectin-3

Abstract

Carrageenans are linear sulfated galactans synthesized in the Gigartinales, Rhodophyceae species with a varied range of biological properties that are of value to the pharmaceutical and cosmetic sectors. It is unknown how the fine structure of carrageenans dictates their capacity to affect molecular and cellular responses important to wound healing, or the ability to mitigate oxidative, hemostatic and inflammatory processes. Here we use specific endo-carrageenases from the marine bacterium Zobellia galactanivorans, to produce enzymatically defined neo-series oligosaccharides from carrageenans. Further enzymatic modification of the oligosaccharides was done by treating with the 3,6-anhydro-Dgalactosidases from the same bacterium which hydrolyse non-reducing end 3,6-anhydro-Dgalactose moieties from neo-carrageenan oligosaccharides. Using the enzymatically produced oligosaccharides, we demonstrate binding to natural human serum antibodies and a monoclonal anti-αGal Ab (m86). The significant interactions with the Galα(1,3)Gal reactive antibodies produced by humans makes them potential potent inducers of complementdependent reactions and attractive for therapeutic applications. We also demonstrate modulation of the galectin selectivity for the Gal-3 Carbohydrate Recognition Domain (CRD) relative to Gal-1 which has implications to targeting specific biological pathways regulated by the galectins.
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Dates and versions

hal-04700919 , version 1 (18-09-2024)

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Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, et al.. Enzymatically-derived oligo-carrageenans interact with α-Gal antibodies and Galectin-3. Carbohydrate Polymers, 2024, 324, pp.121563. ⟨10.1016/j.carbpol.2023.121563⟩. ⟨hal-04700919⟩
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