Over the last two decades, the diffusion of new eating habits and the increase of fish products demand lead to a raising risk for consumers due to fish-borne parasitic zoonosis. Cryptocotyle (Lühe, 1899) are present in marine fish species. Human may be infected by consumption of raw or undercooked fish products. Characterized as zoonotic by Chai and Jung (2017) based on Babbott et al. (1961) human case description in 1999, its impact on human health is still unclear. For the first time, this study intends to characterize Cryptocotyle zoonotic potential. Beforehand conditions of conservation of Cryptocotyle metacercariae were set to organize scheduling of isolation of metacercariae from fish. Then, in vivo trials were performed on mice. After ingestion of 500 Cryptocotyle ligua metacercariae per mouse, daily monitoring of mice was undertaken by observation of their behavior, weight record and fecal time report. Mice were sacrificed twenty-one days post infection and organs were collected for observations of parasites or lesions. This survey constitutes the first analysis of Cryptocotyle lingua zoonotic potential by in vivo experiments. References: Babbott, F. L. J. , Frye, W. W., & Gordon, J. E. (1961). Intestinal parasites of man in Artic Greenland. The American journal of tropical medicine and hygiene, 10, 185-190. Chai, J. Y., & Jung, B.-K. (2017). Fishborne zoonotic heterophyid infections: An update. Food and Waterborne Parasitology, 8-9, 33-63. Acknowledgements: The authors want to thank the Hauts-de-France region of France for financial support.