BACKGROUND AND AIM[|]The exposome encompasses all environmental factors encountered over the life-course to explain their role in the development of health effects, in complement of the genome. Since the emergence of the concept in 2005, the scientific community has made a lot of efforts to produce new knowledge, data, methods and tools to assess the exposome and its impact on human health. However, the exposome has not yet been widely implemented by health and safety agencies and today, one of the key challenges is to include exposome-inspired approaches in health risk assessment.[¤]METHOD[|]After presenting the main recommendations to integrate the exposome in the risk assessment process, we will focus on chemical mixtures to practically show how the exposome can support risk assessment methodologies.[¤]RESULTS[|]Several applications of the different exposure and health data derived from studies in the French population will be presented: dietary exposure to chemical mixtures in children and women, exposure to pesticide mixtures from multiple sources of the general population and from occupational activity for agricultural workers, analysis of chemical mixtures from human biomonitoring data.[¤]CONCLUSIONS[|]The integration of approaches and knowledge relating to the exposome will enable environmental epidemiology and risk assessment to cross a decisive threshold, taking into account the contributions of citizen and participatory science wherever possible and useful. Developing the eco-exposome will also help for a better integration of human, animals and environmental health.