The nonadiabatic molecular alignment effect is commonly found helpful in enhancing nonlinear processes such as high-order harmonics and strong-field ionization. In this work, an opposite phenomenon is observed, in which the nonadiabatic molecular alignment effect prepared with a linearpolarized prepulse strongly suppresses the generation of N$_2$ lasing induced by circularly-polarized 800 nm femtosecond laser pulse in filament plasma. The presence of a weak prepulse periodically suppresses the lasing at each revival timing of the rotational wave packet of the N$_2$ molecule. The underlying mechanism of the lasing signal suppression is attributed to sudden change of the polarization ellipticity of the pump laser pulse. Theoretical simulations by numerically solving the time-dependent Schrödinger equation with the molecular alignment effect included confirm sensitive changes of the polarization ellipticity of the pump laser at every alignment revival, which largely supports our interpretation.