Complete sequence of transferable IncHI2/ST4 plasmid carrying blaCTX-M-1 and mcr-1 from an enterohaemorrhagic Escherichia coli (EHEC) from bovine origin
Résumé
The co-occurrence of Extended-Spectrum Beta-Lactamase (ESBL) and mcr-1 encoding genes on plasmids have been recently demonstrated on a single cell. After finding a co-localization of these antimicrobial genes on a single plasmid here, we show the complete annotated sequence of a large plasmid encoding both blaCTX-M-1 and mcr-1 genes isolated from an enterohaemorrhagic E. coli (EHEC) isolate. The genomic features of this plasmid were analysed in order to provide information to better control its spread. The aim of this study is to provide complete genome of a plasmid carrying both blaCTX-M-1 and mcr-1 from EHEC. Methods: Whole Genome Sequencing of transconjugant containing the single plasmid carrying both blaCTXM-1 and mcr-1 isolated from EHEC. Results: This study reports the complete sequence of a transferable mcr-1/blaCTX-M-1 bearing IncHI2/ST4 plasmid harboring numerous virulence factors and resistance determinants to seven antibiotic classes, for the first time isolated from an ESBL-producing EHEC. Conclusions: IncHI2/ST4 plasmids were already shown to disseminate blaCTX-M-1 and mcr-1 in cattle E. coli in France and this data now highlights their spread among enterohaemorrhagic E. coli at risk for public health.
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