Article Dans Une Revue International Journal of Radiation Biology Année : 2024

In vitro validation of helium ion irradiations as a function of linear energy transfer in radioresistant human malignant cells

Aleksandra M. Ristić Fira
  • Fonction : Auteur
Otilija D Keta
  • Fonction : Auteur
Vladana D Petković
  • Fonction : Auteur
Miloš Đorđević
  • Fonction : Auteur
Giada Petringa
  • Fonction : Auteur
Serena Fattori
  • Fonction : Auteur
Roberto Catalano
  • Fonction : Auteur
Giuseppe Pablo Cirrone
  • Fonction : Auteur
Giacomo Cuttone
  • Fonction : Auteur
Dousatsu Sakata
  • Fonction : Auteur
Konstantinos Chatzipapas
  • Fonction : Auteur
Ivan M Petrović
  • Fonction : Auteur

Résumé

PurposeBased on considerable interest to enlarge the experimental database of radioresistant cells after their irradiation with helium ions, HTB140, MCF-7 and HTB177 human malignant cells are exposed to helium ion beams having different linear energy transfer (LET).Materials and methodsThe cells are irradiated along the widened 62 MeV/u helium ion Bragg peak, providing LET of 4.9, 9.8, 23.4 and 36.8 keV/mu m. Numerical simulations with the Geant4 toolkit are used for the experimental design. Cell survival is evaluated and compared with reference gamma-rays. DNA double strand breaks are assessed via gamma-H2AX foci.ResultsWith the increase of LET, surviving fractions at 2 Gy decrease, while RBE (2 Gy, gamma) gradually increase. For HTB140 cells, above the dose of 4 Gy, a slight saturation of survival is observed while the increase of RBE (2 Gy, gamma) remains unaffected. With the increase of LET the increase of gamma-H2AX foci is revealed at 0.5 h after irradiation. There is no significant difference in the number of foci between the cell lines for the same LET. From 0.5 to 24 h, the number of foci drops reaching its residual level. For each time point, there are small differences in DNA DSB among the three cell lines.ConclusionAnalyses of data acquired for the three cell lines irradiated by helium ions, having different LET, reveal high elimination capacity and creation of a large number of DNA DSB with respect to gamma-rays, and are between those reported for protons and carbon ions.

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hal-04668737 , version 1 (24-10-2024)

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Aleksandra M. Ristić Fira, Otilija D Keta, Vladana D Petković, Miloš Đorđević, Giada Petringa, et al.. In vitro validation of helium ion irradiations as a function of linear energy transfer in radioresistant human malignant cells. International Journal of Radiation Biology, 2024, 100 (10), pp.1426-1437. ⟨10.1080/09553002.2024.2373752⟩. ⟨hal-04668737⟩
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