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Article Dans Une Revue Science Advances Année : 2024

Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni

Sonja Rose
Brent Robicheau
Jennifer Tolman
Debany Fonseca-Batista
Elden Rowland
Dhwani Desai
Ella Joy H. Kantor
André Comeau
Morgan G.I. Langille
Jon Jerlström-Hultqvist
Erin Bertrand
Julie Laroche

Résumé

The high diversity and global distribution of heterotrophic bacterial diazotrophs (HBDs) in the ocean has recently become apparent. However, understanding the role these largely uncultured microorganisms play in marine N 2 fixation poses a challenge due to their undefined growth requirements and the complex regulation of the nitrogenase enzyme. We isolated and characterized Candidatus Thalassolituus haligoni, a member of a widely distributed clade of HBD belonging to the Oceanospirillales. Analysis of its nifH gene via amplicon sequencing revealed the extensive distribution of Cand. T. haligoni across the Pacific, Atlantic, and Arctic Oceans. Pangenome analysis indicates that the isolate shares >99% identity with an uncultured metagenome-assembled genome called Arc-Gamma-03, recently recovered from the Arctic Ocean. Through combined genomic, proteomic, and physiological approaches, we confirmed that the isolate fixes N 2 gas. However, the mechanisms governing nitrogenase regulation in Cand. T. haligoni remain unclear. We propose Cand. T. haligoni as a globally distributed, cultured HBD model species within this understudied clade of Oceanospirillales.
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Dates et versions

hal-04666250 , version 1 (01-08-2024)

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Sonja Rose, Brent Robicheau, Jennifer Tolman, Debany Fonseca-Batista, Elden Rowland, et al.. Nitrogen fixation in the widely distributed marine γ-proteobacterial diazotroph Candidatus Thalassolituus haligoni. Science Advances , 2024, 10 (31), pp.eadn1476. ⟨10.1126/sciadv.adn1476⟩. ⟨hal-04666250⟩
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