In vitro Study of Lactobacillus paracasei CNCM I-1518 in Healthy and Clostridioides difficile Colonized Elderly Gut Microbiota - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Frontiers in Nutrition Année : 2019

In vitro Study of Lactobacillus paracasei CNCM I-1518 in Healthy and Clostridioides difficile Colonized Elderly Gut Microbiota

Sophie Fehlbaum
  • Fonction : Auteur
Clarissa Schwab
  • Fonction : Auteur
Maarja Voolaid
  • Fonction : Auteur
Candice Fourmestraux
  • Fonction : Auteur
Muriel Derrien
  • Fonction : Auteur
Christophe Lacroix
  • Fonction : Auteur

Résumé

Consumption of probiotic bacteria can result in a transient colonization of the human gut and thereby in potential interactions with the commensal microbiota. In this study, we used novel PolyFermS continuous fermentation models to investigate interactions of the candidate probiotic strain Lactobacillus paracasei CNCM I-1518 (L. paracasei) with colonic microbiota from healthy elderly subjects using 16S rRNA gene amplicon sequencing and metatranscriptomics, or with microbiota in vitro-colonized with Clostridioides difficile (C. difficile NCTC 13307 and C. difficile DSM 1296)—an enteropathogen prevalent in the elderly population. Small changes in microbiota composition were detected upon daily addition of L. paracasei, including increased abundances of closely related genera Lactobacillus and Enterococcus, and of the butyrate producer Faecalibacterium. Microbiota gene expression was also modulated by L. paracasei with distinct response of the Faecalibacterium transcriptome and an increase in carbohydrate utilization. However, no inhibitory effect of L. paracasei was observed on C. difficile colonization in the intestinal models under the tested conditions. Our data suggest that, in the in vitro experimental conditions tested and independent of the host, L. paracasei has modulatory effects on both the composition and function of elderly gut microbiota without affecting C. difficile growth and toxin production.

Dates et versions

hal-04660909 , version 1 (24-07-2024)

Identifiants

Citer

Sophie Fehlbaum, Christophe Chassard, Clarissa Schwab, Maarja Voolaid, Candice Fourmestraux, et al.. In vitro Study of Lactobacillus paracasei CNCM I-1518 in Healthy and Clostridioides difficile Colonized Elderly Gut Microbiota. Frontiers in Nutrition, 2019, 6, ⟨10.3389/fnut.2019.00184⟩. ⟨hal-04660909⟩
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