Metabolite Profiling and Molecular Network Shows Kinkeloids as promoting of collagen synthesis from Combretum micranthum
Résumé
This presentation will investigate the molecular composition and a new cosmetically relevant biological activity of Combretum micranthum extract and enriched fractions to begin to establish a structure-activity relationship. Combretum micranthum, a plant native to Africa, has a well-documented traditional use in the treatment of various ailments such as fever, diabetes and malaria (Yoro, Tine et al., 2024). Its pharmaceutical benefits include nephroprotective, anti-inflammatory, antioxidant and antimicrobial properties have been demonstrated (Olajide, Olumayokun et al., 2003). In addition, its potential for cosmetic applications is being explored due to its depigmenting, anti- inflammatory and UV damage repairing properties (胡舒婷 et al., 2020). First, an extract of Combretum micranthum was prepared and selected for its overall biological response, then fractionated to obtain simplified molecular fractions. One fraction was particularly enriched in kinkeloids, a family of compounds specific to this species. All fractions and the crude extract were then tested on biological targets to evaluate and compare their cosmetic activities. Molecular networks were constructed from the UHPLC-MS/HRMS data to better characterize the extract and fractions and to highlight structure-activity relationships. This study highlights the metabolic profiling of a butylene glycol extract of Combretum micranthum, showing its main chemical families and revealing that the kinkeloids identified by HRMS and NMR promote an increase in collagen I synthesis, an interesting cosmetic activity not previously described for these compounds or for any Combretum micranthum extract.