In vitro physiological effects and phytochemical exploration of four Sideritis L. species from Türkiye.
Résumé
Sideritis L. is a member of the Lamiaceae family. There are approximately 218 taxa in the world and 60 taxa in Türkiye belonging to the Sideritis genus. Among these 60 taxa, 40 are endemic to Türkiye (Duman, 2012; WFO, 2024). According to ethnobotanical studies, Sideritis species have been used in the treatment of skin
disease, cold, cough, wound, diarrhoea (Tuzlacı, 2016). The Sideritis genus has a wide range of chemical components, including lignans, sterols, iridoids, terpenoids and flavonoids (González-Burgos, 2011).
Four different Sideritis taxa were collected for this study from different localities and after identification stored at Herbarium of Altınbaş University, Faculty of Pharmacy (HERA). The ethanolic extracts of these four species were tested for their cytotoxicity on L929 fibroblast cells and their metabolic activities on
keratinocyte cells (HaCat) and endothelial cells (HuVeC) in order to better understand their uses. Sideritis congesta P.H.Davis & Hub.-Mor. and Sideritis montana L. have proven ineffective on keratinocytes but demonstrated activation of dermal cells. Intriguingly, Sideritis trojana Bornm. selectively promote the
division of HuVeC cells, while Sideritis germanicopolitana subsp. germanicopolitana Bornm. stimulate a more significant proliferation of fibroblast cells compared to the negative control group.
Extracts have been analysed by UHPLC-DAD-ELSD and are mainly composed by flavonoids and terpenoids. Analysis have shown a large difference in terpenoids composition. UHPLC-HRMS/MS analysis are in progress to refine the compound identification and better understand the differences of physiological effects of these extracts.