The spread of livestock activities and the expansion of wild ungulates populations favor the share of grazing pastures among species, increasing opportunities for direct or indirect cross-transmission of pathogens. While most studies focused on major economic and public-health infectious diseases, comparatively few studies focused on the epidemiology of gastro-intestinal nematode (GIN) parasites at the wildlife/livestock interface. These parasites are ubiquitous and of major concerns for both domestic and wild ungulates, as they may cause disease or loss of productivity. Although breeders can use anthelmintic drugs to control the infestation of livestock by gastrointestinal nematodes to limit diseases and loss of productivity, those parasites are also hosted by wildlife which can contribute to their circulation. In this study, we aimed to assess the influence of sharing pastures with livestock on the parasitism of roe deer in southern France. Using DNA metabarcoding of ITS-2 rDNA from larvae isolated from fecal samples, we analyzed the gastrointestinal nematodes communities (termed “nemabiome”) of roe deer and sheep inhabiting a heterogeneous rural landscape. While the dominant GIN species found in roe deer and sheep are generalist species (Haemonchus contortus, Trichostrongylus axei), we also detected in wild roe deer GIN species usually found in wild cervids (e.g., Ostertagia leptospicularis, Spiculopteragia spp.), but at low frequency. Nemabiome in roe deer varied along the sampling period, revealing seasonal variation of gastrointestinal species in this species. In addition, we detected a low variation of nemabiome related to the proximity of roe deer samples to livestock pastures. Our results suggest exchanges of gastrointestinal nematodes between and within domestic and wild ungulates in this rural landscape. It emphasizes the role of domestic ungulates in contaminating wild species, but also the potential role of wild ungulates as reservoirs and spreaders of parasites −including drug resistant parasites− between locations.