Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo - Archive ouverte HAL
Article Dans Une Revue Scientific Reports Année : 2018

Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo

Simon Bucher
  • Fonction : Auteur
Normand Podechard
  • Fonction : Auteur
Marie Liamin
  • Fonction : Auteur
Dounia Le Guillou
  • Fonction : Auteur
Martine Chevanne
  • Fonction : Auteur
Cédric Coulouarn
  • Fonction : Auteur
Muhammad Imran
  • Fonction : Auteur
Isabelle Gallais
  • Fonction : Auteur
Morgane Fernier
  • Fonction : Auteur
Quentin Hamdaoui
  • Fonction : Auteur
Marie-Anne Robin
  • Fonction : Auteur
Odile Sergent
  • Fonction : Auteur
Bernard Fromenty
  • Fonction : Auteur
Dominique Lagadic-Gossmann
  • Fonction : Auteur

Résumé

Abstract Hepatic steatosis (i.e. lipid accumulation) and steatohepatitis have been related to diverse etiologic factors, including alcohol, obesity, environmental pollutants. However, no study has so far analyzed how these different factors might interplay regarding the progression of liver diseases. The impact of the co-exposure to the environmental carcinogen benzo[a]pyrene (B[a]P) and the lifestyle-related hepatotoxicant ethanol, was thus tested on in vitro models of steatosis (human HepaRG cell line; hybrid human/rat WIF-B9 cell line), and on an in vivo model (obese zebrafish larvae). Steatosis was induced prior to chronic treatments (14, 5 or 7 days for HepaRG, WIF-B9 or zebrafish, respectively). Toxicity and inflammation were analyzed in all models; the impact of steatosis and ethanol towards B[a]P metabolism was studied in HepaRG cells. Cytotoxicity and expression of inflammation markers upon co-exposure were increased in all steatotic models, compared to non steatotic counterparts. A change of B[a]P metabolism with a decrease in detoxification was detected in HepaRG cells under these conditions. A prior steatosis therefore enhanced the toxicity of B[a]P/ethanol co-exposure in vitro and in vivo ; such a co-exposure might favor the appearance of a steatohepatitis-like state, with the development of inflammation. These deleterious effects could be partly explained by B[a]P metabolism alterations.

Dates et versions

hal-04627121 , version 1 (27-06-2024)

Identifiants

Citer

Simon Bucher, Arnaud Tête, Normand Podechard, Marie Liamin, Dounia Le Guillou, et al.. Co-exposure to benzo[a]pyrene and ethanol induces a pathological progression of liver steatosis in vitro and in vivo. Scientific Reports, 2018, 8 (1), pp.5963. ⟨10.1038/s41598-018-24403-1⟩. ⟨hal-04627121⟩
6 Consultations
0 Téléchargements

Altmetric

Partager

More