VCA supercooling in a swine partial hindlimb model
Yanis Berkane
(1, 2, 3, 4, 5)
,
Irina Filz von Reiterdank
(2, 6, 4)
,
Pierre Tawa
(2, 3, 4)
,
Laura Charlès
(2, 3, 4)
,
Marion Goutard
(1, 2, 3, 4)
,
Antonia T. Dinicu
(2, 3, 4)
,
Mehmet Toner
(2, 3, 4)
,
Nicolas Bertheuil
(1, 5)
,
Aebele B. Mink van der Molen
(6)
,
J. Henk Coert
(6)
,
Alexandre G Lellouch
(7, 2, 4, 3)
,
Mark A. Randolph
(2, 4, 3)
,
Curtis L. Cetrulo Jr
(2, 4, 3)
,
Korkut Uygun
(2, 4, 3)
1
MOBIDIC -
Microenvironment and B-cells: Immunopathology,Cell Differentiation, and Cancer
2 Shriners Hospitals for Children [Boston]
3 Massachusetts General Hospital [Boston]
4 HMS - Harvard Medical School [Boston]
5 Hôpital Sud [CHU Rennes]
6 UMCU - University Medical Center [Utrecht]
7 IThEM - U1140 - Innovations thérapeutiques en hémostase = Innovative Therapies in Haemostasis
2 Shriners Hospitals for Children [Boston]
3 Massachusetts General Hospital [Boston]
4 HMS - Harvard Medical School [Boston]
5 Hôpital Sud [CHU Rennes]
6 UMCU - University Medical Center [Utrecht]
7 IThEM - U1140 - Innovations thérapeutiques en hémostase = Innovative Therapies in Haemostasis
Pierre Tawa
- Fonction : Auteur
- PersonId : 1326018
- ORCID : 0000-0002-5801-7505
Nicolas Bertheuil
- Fonction : Auteur
- PersonId : 947305
- ORCID : 0000-0002-4602-0432
J. Henk Coert
- Fonction : Auteur
- PersonId : 1325951
- ORCID : 0000-0002-1921-9737
Mark A. Randolph
- Fonction : Auteur
- PersonId : 1325952
- ORCID : 0000-0003-0877-9832
Curtis L. Cetrulo Jr
- Fonction : Auteur
- PersonId : 1325953
- ORCID : 0000-0001-7080-3894
Korkut Uygun
- Fonction : Auteur correspondant
Résumé
Vascularized composite allotransplantations are complex procedures with substantial functional impact on patients. Extended preservation of VCAs is of major importance in advancing this field. It would result in improved donor-recipient matching as well as the potential for ex vivo manipulation with gene and cell therapies. Moreover, it would make logistically feasible immune tolerance induction protocols through mixed chimerism. Supercooling techniques have shown promising results in multi-day liver preservation. It consists of reaching sub-zero temperatures while preventing ice formation within the graft by using various cryoprotective agents. By drastically decreasing the cell metabolism and need for oxygen and nutrients, supercooling allows extended preservation and recovery with lower ischemia-reperfusion injuries. This study is the first to demonstrate the supercooling of a large animal model of VCA. Porcine hindlimbs underwent 48 h of preservation at - 5 degrees C followed by recovery and normothermic machine perfusion assessment, with no issues in ice formation and favorable levels of injury markers. Our findings provide valuable preliminary results, suggesting a promising future for extended VCA preservation.
Domaines
CancerFormat du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
VCA supercooling in a swine partial hindlimb model
|
Résumé |
en
Vascularized composite allotransplantations are complex procedures with substantial functional impact on patients. Extended preservation of VCAs is of major importance in advancing this field. It would result in improved donor-recipient matching as well as the potential for ex vivo manipulation with gene and cell therapies. Moreover, it would make logistically feasible immune tolerance induction protocols through mixed chimerism. Supercooling techniques have shown promising results in multi-day liver preservation. It consists of reaching sub-zero temperatures while preventing ice formation within the graft by using various cryoprotective agents. By drastically decreasing the cell metabolism and need for oxygen and nutrients, supercooling allows extended preservation and recovery with lower ischemia-reperfusion injuries. This study is the first to demonstrate the supercooling of a large animal model of VCA. Porcine hindlimbs underwent 48 h of preservation at - 5 degrees C followed by recovery and normothermic machine perfusion assessment, with no issues in ice formation and favorable levels of injury markers. Our findings provide valuable preliminary results, suggesting a promising future for extended VCA preservation.
|
Auteur(s) |
Yanis Berkane
1, 2, 3, 4, 5
, Irina Filz von Reiterdank
2, 6, 4
, Pierre Tawa
2, 3, 4
, Laura Charlès
2, 3, 4
, Marion Goutard
1, 2, 3, 4
, Antonia T. Dinicu
2, 3, 4
, Mehmet Toner
2, 3, 4
, Nicolas Bertheuil
1, 5
, Aebele B. Mink van der Molen
6
, J. Henk Coert
6
, Alexandre G Lellouch
7, 2, 4, 3
, Mark A. Randolph
2, 4, 3
, Curtis L. Cetrulo Jr
2, 4, 3
, Korkut Uygun
2, 4, 3
1
MOBIDIC -
Microenvironment and B-cells: Immunopathology,Cell Differentiation, and Cancer
( 1084051 )
- Université de Rennes
- France
2
Shriners Hospitals for Children [Boston]
( 418581 )
- 51 Blossom St.
Boston, MA 02114
Massachusetts
- États-Unis
3
Massachusetts General Hospital [Boston]
( 309442 )
- 55 Fruit Street
Boston, MA 02114
- États-Unis
4
HMS -
Harvard Medical School [Boston]
( 130467 )
- 25 Shattuck Street Boston, MA 02115
- États-Unis
5
Hôpital Sud [CHU Rennes]
( 1057398 )
- CHU de Rennes
- France
6
UMCU -
University Medical Center [Utrecht]
( 73956 )
- Heidelberglaan 100
3584 CX Utrecht
- Pays-Bas
7
IThEM - U1140 -
Innovations thérapeutiques en hémostase = Innovative Therapies in Haemostasis
( 1004816 )
- Site : Faculté des Sciences Pharmaceutiques et biologiques, 4 avenue de l'Observatoire 75270 Paris Cedex 06
Site de Hôpital Européen Georges-Pompidou, 20 rue Leblanc 75015 Paris
- France
|
Langue du document |
Anglais
|
Nom de la revue |
|
Volume |
14
|
Numéro |
1
|
Page/Identifiant |
12618
|
Date de publication |
2024
|
Audience |
Internationale
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Licence |
Paternité
|
Domaine(s) |
|
Financement |
|
DOI | 10.1038/s41598-024-63041-8 |
Pubmed Id | 38824189 |
PubMed Central | PMC11144209 |
Origine :
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