High throughput microfluidic system for separation and quantification of high-molecular-weight glutenin subunits
Résumé
Knowledge of glutenin-subunit composition is important for the prediction of the genetic potential of breeding lines because these polypeptides are known to be responsible for the main differences in breadmaking quality. High-molecular-weight glutenin subunits (HMW-GS) which are especially important for quality screening were commonly identified by SDS gel electrophoresis, reversed-phase high-performance chromatography (RP-HPLC) or capillary electrophoresis. Even if automatic sampling improves the efficiency of these techniques, high throughput analytic procedure must be developped. In this sudy, the use of a new Lab-on-Chip platform has been explored for a high speed qualitative and quantitative analysis of HMW-GS. 130 French common wheat varieties were used for the identification and the allocation of individual HMW-GS to Lab-on-a-Chip profiles. Furthermore, all the HMW-GS were individually quantified for genotype comparaison. As shows it the results obtained, the Lab-on-a-Chip platform used offers a unique high throughput (a 96 well plate can be analysed in a little over one hour) procedure with high resolution and high sensitivity. The microfluidic chips we evaluated appeared highy reproducible. This high throughput method appears to be extremely well suited for routine identification and quantification of HMW-GS especially for puposes of wheat quality screening and wheat cultivar development activities where large numbers of samples are typically encountered.
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