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Article Dans Une Revue Chemistry - A European Journal Année : 2020

Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes

Résumé

Abstract The 10–23 DNAzyme is an artificially developed Mg 2+ ‐dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence‐specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg 2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best‐performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.

Domaines

Chimie

Dates et versions

hal-04592503 , version 1 (29-05-2024)

Identifiants

Citer

Mégane Debiais, Amandine Lelievre, Jean‐jacques Vasseur, Sabine Müller, Michael Smietana. Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes. Chemistry - A European Journal, 2020, 27 (3), pp.1138-1144. ⟨10.1002/chem.202004227⟩. ⟨hal-04592503⟩
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