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Article Dans Une Revue Chemistry - A European Journal Année : 2020

Boronic acid‐mediated activity control of split 10‐23 DNAzymes

Mégane Debiais
  • Fonction : Auteur
Lelievre Amandine
  • Fonction : Auteur
Sabine Müller
  • Fonction : Auteur
Michaël Smietana

Résumé

The 10-23 DNAzyme is an artificially developed Mg2+-dependent catalytic oligonucleotide that can cleave a RNA substrate in a sequence-specific fashion. In this study, we designed new split 10-23 DNAzymes made of two non-functional fragments, the first one carrying a boronic acid group at its 5' end, while the other one is composed of a ribonucleotide at its 3' end. We demonstrate herein that the addition of Mg2+ ions leads to the assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation has been performed that identified the most performant system. Our results highlight key features for an efficient control of DNAzymes activity through the formation of boronate linkages.
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Dates et versions

hal-02992069 , version 1 (06-11-2020)

Identifiants

Citer

Mégane Debiais, Lelievre Amandine, Jean-Jacques Vasseur, Sabine Müller, Michaël Smietana. Boronic acid‐mediated activity control of split 10‐23 DNAzymes. Chemistry - A European Journal, In press, ⟨10.1002/chem.202004227⟩. ⟨hal-02992069⟩
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