Article Dans Une Revue Physical Review Letters Année : 2010

Scraping and Stapling of End-Grafted DNA Chains by a Bioadhesive Spreading Vesicle to Reveal Chain Internal Friction and Topological Complexity

Gi-Moon Nam
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Marie Laure Hisette
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Yuting Liang Sun
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Thomas Gisler
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Albert Johner
Fabrice Thalmann
André Schroder
Carlos Marques
Nam-Kyung Lee
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Résumé

Stained end-grafted DNA molecules about 20  μm long are scraped away and stretched out by the spreading front of a bioadhesive vesicle. Tethered biotin ligands bind the vesicle bilayer to a streptavidin substrate, stapling the DNAs into frozen confinement paths. Image analysis of the stapled DNA gives access, within optical resolution, to the local stretching values of individual DNA molecules swept by the spreading front, and provides evidence of self-entanglements.
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Dates et versions

hal-04577101 , version 1 (15-05-2024)

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Gi-Moon Nam, Marie Laure Hisette, Yuting Liang Sun, Thomas Gisler, Albert Johner, et al.. Scraping and Stapling of End-Grafted DNA Chains by a Bioadhesive Spreading Vesicle to Reveal Chain Internal Friction and Topological Complexity. Physical Review Letters, 2010, 105 (8), ⟨10.1103/PhysRevLett.105.088101⟩. ⟨hal-04577101⟩
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