A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche - Archive ouverte HAL
Journal Articles Development (Cambridge, England) Year : 2024

A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche

Abstract

The first hematopoietic stem and progenitor cells (HSPCs) emerge in the Aorta-Gonad-Mesonephros (AGM) region of the mid-gestation mouse embryo. However, the precise nature of their supportive mesenchymal microenvironment remains largely unexplored. Here, we profiled transcriptomes of laser micro-dissected aortic tissues at three developmental stages and individual AGM cells. Computational analyses allowed the identification of several cell subpopulations within the E11.5 AGM mesenchyme, with the presence of a yet unidentified subpopulation characterized by the dual expression of genes implicated in adhesive or neuronal functions. We confirmed the identity of this cell subset as a neuro-mesenchymal population, through morphological and lineage tracing assays. Loss of function in the zebrafish confirmed that Decorin, a characteristic extracellular matrix component of the neuro-mesenchyme, is essential for HSPC development. We further demonstrated that this cell population is not merely derived from the neural crest, and hence, is a bona fide novel subpopulation of the AGM mesenchyme.
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Dates and versions

hal-04576520 , version 1 (04-11-2024)

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Olivera Miladinovic, Pierre-Yves Canto, Claire Pouget, Olivier Piau, Nevenka Radic, et al.. A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche. Development (Cambridge, England), 2024, 151 (7), ⟨10.1242/dev.202614⟩. ⟨hal-04576520⟩
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