Genetic control of root morphology in response to nitrogen across rapeseed diversity - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Physiologia Plantarum Année : 2024

Genetic control of root morphology in response to nitrogen across rapeseed diversity

Julien Louvieaux
Claudia Chiodi
  • Fonction : Auteur
  • PersonId : 1120473
László Kupcsik
Andreas Stahl

Résumé

Rapeseed (Brassica napus L.) is an oil‐containing crop of great economic value but with considerable nitrogen requirement. Breeding root systems that efficiently absorb nitrogen from the soil could be a driver to ensure genetic gains for more sustainable rapeseed production. The aim of this study is to identify genomic regions that regulate root morphology in response to nitrate availability. The natural variability offered by 300 inbred lines was screened at two experimental locations. Seedlings grew hydroponically with low or elevated nitrate levels. Fifteen traits related to biomass production and root morphology were measured. On average across the panel, a low nitrate level increased the root‐to‐shoot biomass ratio and the lateral root length. A large phenotypic variation was observed, along with important heritability values and genotypic effects, but low genotype‐by‐nitrogen interactions. Genome‐wide association study and bulk segregant analysis were used to identify loci regulating phenotypic traits. The first approach nominated 319 SNPs that were combined into 80 QTLs. Three QTLs identified on the A07 and C07 chromosomes were stable across nitrate levels and/or experimental locations. The second approach involved genotyping two groups of individuals from an experimental F2 population created by crossing two accessions with contrasting lateral root lengths. These individuals were found in the tails of the phenotypic distribution. Co‐localized QTLs found in both mapping approaches covered a chromosomal region on the A06 chromosome. The QTL regions contained some genes putatively involved in root organogenesis and represent selection targets for redesigning the root morphology of rapeseed.
Fichier non déposé

Dates et versions

hal-04576276 , version 1 (15-05-2024)

Identifiants

Citer

Loïc Haelterman, Julien Louvieaux, Claudia Chiodi, Anne-Sophie Bouchet, László Kupcsik, et al.. Genetic control of root morphology in response to nitrogen across rapeseed diversity. Physiologia Plantarum, 2024, 176 (3), pp.e14315. ⟨10.1111/ppl.14315⟩. ⟨hal-04576276⟩
0 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More