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Article Dans Une Revue Sensors and Actuators B: Chemical Année : 2021

Quinolimide-based peptide biosensor for probing p25 in vitro and in living cells

Résumé

CDK5 kinase is activated through interactions with different partners including the p35/p25 regulators. Active CDK5 plays a key role in several neuronal functions and its hyperactivity contributes to a variety of neurodegenerative processes and several human cancers, in particular glioblastomas and neuroblastomas. In order to probe partners that interact with CDK5, we designed and synthesized fluorescent quinolimide-labelled peptides derived from the C-helix of CDK5, which were implemented to probe CDK5 partners in vitro and by in cellulo imaging in U87 glioblastoma and N2a neuroblastoma cells. Ectopic expression of a NIR fluorescent protein miRFP670 fusion with p25 (p25-miRFP670) in N2a cells induced FRET between a quinolimide-labelled peptide biosensor and p25-miRFP670, and fluorescence intensity was proportional to the expression level of p25, thereby demonstrating the potential of the quinolimide-labelled peptide biosensor to report on p25 relative abundance.

Domaines

Chimie

Dates et versions

hal-04570407 , version 1 (07-05-2024)

Identifiants

Citer

Francisco Fueyo-González, Rosario Herranz, Simona Plesselova, Maria Giron, Rafael Salto, et al.. Quinolimide-based peptide biosensor for probing p25 in vitro and in living cells. Sensors and Actuators B: Chemical, 2021, 339, pp.129929. ⟨10.1016/j.snb.2021.129929⟩. ⟨hal-04570407⟩
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