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Article Dans Une Revue Biotechniques Année : 2024

Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information

Résumé

In the absence of a DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. In this report we propose a fully in vitro protocol to generate very long double-stranded DNA molecules starting from commercially available short DNA blocks in less than 3 days using Golden Gate assembly. This innovative application allowed us to streamline the process to produce a 24 kb-long DNA molecule storing part of the  Declaration of the Rights of Man and of the Citizen of 1789 . The DNA molecule produced can be readily cloned into a suitable host/vector system for amplification and selection.

Dates et versions

hal-04567229 , version 1 (03-05-2024)

Identifiants

Citer

Julien Leblanc, Olivier Boulle, Emeline Roux, Jacques Nicolas, Dominique Lavenier, et al.. Fully in vitro iterative construction of a 24 kb-long artificial DNA sequence to store digital information. Biotechniques, 2024, 76 (5), pp.203-215. ⟨10.2144/btn-2023-0109⟩. ⟨hal-04567229⟩
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