Trichinella spiralis inhibits myoblast differentiation by targeting SQSTM1/p62 with a secreted E3 ubiquitin ligase - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue iScience Année : 2024

Trichinella spiralis inhibits myoblast differentiation by targeting SQSTM1/p62 with a secreted E3 ubiquitin ligase

Jian Da Pang
  • Fonction : Auteur
Xue Min Jin
  • Fonction : Auteur
Yi Liu
  • Fonction : Auteur
Zi Jian Dong
  • Fonction : Auteur
Jing Ding
  • Fonction : Auteur
Ming Yuan Liu
  • Fonction : Auteur
Ning Xu
  • Fonction : Auteur
Xiao Lei Liu

Résumé

Trichinella spiralis infection is associated with the formation of cysts within host skeletal muscle cells, thereby enabling immune evasion and subsequent growth and development; however, the pathogenic factors involved in this process and their mechanisms remain elusive. Here, we found that Ts-RNF secreted by T. spiralis is required for its growth and development in host cells. Further study revealed that Ts-RNF functions as an E3 ubiquitin ligase that targets the UBA domain of SQSTM1/p62 by forming K63-type ubiquitin chains. This modification interferes with autophagic flux, leading to impaired mitochondrial clearance and abnormal myotube differentiation and fusion. Our results established that T. spiralis increases its escape by interfering with host autophagy via the secretion of an E3 ubiquitin ligase.

Dates et versions

hal-04558027 , version 1 (24-04-2024)

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Citer

Jian Da Pang, Xue Min Jin, Yi Liu, Zi Jian Dong, Jing Ding, et al.. Trichinella spiralis inhibits myoblast differentiation by targeting SQSTM1/p62 with a secreted E3 ubiquitin ligase. iScience, 2024, 27 (3), pp.109102. ⟨10.1016/j.isci.2024.109102⟩. ⟨hal-04558027⟩
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