Production of Pseudomonas mycins in microbioreactor: Exploring capacity and scalability - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

Production of Pseudomonas mycins in microbioreactor: Exploring capacity and scalability

Résumé

Biocontrol products such as living organisms or natural substances are sustainable alternatives to conventional pesticides for agriculture. Pseudomonas spp. are known to produce a wide range of lipopeptides which have potential applications in agriculture as biocontrol agents against phytopathogenic bacteria and fungi. Among the lipopeptides of the mycin family, syringomycin E was reported to be a potentially novel biofungicide (Kawasaki et al., 2016). Wild bacterial strains often produced lipopeptides in limited quantity under culture conditions and little research has been done to explore its production potential in bioreactors. To the best of our knowledge, only syringomycin E was reported to be produced in a scalable quantity by a variant of Pseudomonas syringae pv. syringae strain B301D (Kawasaki et al., 2016). In this study, we aimed to investigate the growing strength of a collection of producing strains and to evaluate the production potential of lipopeptides at micro-size bioreactor scale. To achieve this, a collection of 71 Pseudomonas strains previously proved to be able to produce lipopeptides (Bricout et al., 2022) were studied through a combination of a high-throughput cultivation platform and analytical tools. To assess their growth behavior, bacteria were cultured in 48-well FlowerPlate of BioLector (m2p labs, GmbH) allowing multiple-parameter measurement under controlled conditions. The production of mycins was analyzed by HPLC-QDa and the mycin amount was determined by using purified surfactin as standard. The results of our study demonstrated that most bacterial strains had a relatively high biomass production which is a key element leading to superior production of the target compounds. 48% of the tested strains possessed the capacity to synthesize and secrete mycins by feeding on simple culture medium and yielded from 2 to 122 mg/l of mycins. The relatively large production of mycins by a large proportion of strains suggests the potential of Pseudomonas spp. for large-scale mycin production. References Bricout, A., Morris, C. E., Chandeysson, C., Duban, M., Boistel, C., Chataigné, G., Lecouturier, D., Jacques, P., Leclère, V., & Rochex, A. (2022). The Diversity of Lipopeptides in the Pseudomonas syringae Complex Parallels Phylogeny and Sheds Light on Structural Diversification during Evolutionary History. Microbiology Spectrum, e0145622. https://doi.org/10.1128/spectrum.01456-22 Kawasaki, Y., Nischwitz, C., Grilley, M. M., Jones, J., Brown, J. D., & Takemoto, J. Y. (2016). Production and Application of Syringomycin E as an Organic Fungicide Seed Protectant against Pythium Damping-off. Journal of Phytopathology, 164(10), 801–810. https://doi.org/10.1111/jph.12500
Fichier non déposé

Dates et versions

hal-04551532 , version 1 (18-04-2024)

Identifiants

  • HAL Id : hal-04551532 , version 1

Citer

Sorphea Heang, Angeline Pelus, Chloé Dupuis, Cindy E. Morris, Alice Rochex, et al.. Production of Pseudomonas mycins in microbioreactor: Exploring capacity and scalability. 14. European congress of chemical engineering, European Federation of Chemical Engineering (EFCE); European Society of Biochemical Engineering Sciences (ESBES), Sep 2023, Berlin, Germany. ⟨hal-04551532⟩
0 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More