A combined approach of genome mining and in vitro evaluation of Biocontrol and Plant Growth-Promoting Bacteria (PGPB) functions of Pseudomonas strains - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2023

A combined approach of genome mining and in vitro evaluation of Biocontrol and Plant Growth-Promoting Bacteria (PGPB) functions of Pseudomonas strains

Résumé

In recent years, the focus has turned from the use of chemical pesticides and inorganic fertilizers to the development of biological control agents as non-toxic natural options and sustainable management strategies for plant protection. Among these biofertilizers and biopesticides, plant growth promoting bacteria (PGPB) have been reported to enhance plant growth by providing nutrients such as nitrogen, iron, and phosphorus, and protect plants against phytopathogens by the production of bioactive compounds. The aim of this study was to investigate the biocontrol and growth-promoting effects of Pseudomonas strains isolated from water collected from the granitic soil of Vosges (France). A combined approach of bioinformatic genome analysis and in vitro evaluation of related functions was carried out. The whole genome of 3 Pseudomonas strains was sequenced and analyzed to identify biosynthetic gene clusters involved in PGPB functions including (i) phosphate solubilization by searching for its complete machinery like the presence of phosphatase gene and genes encoding for some organic acids; (ii) nitrogen metabolism such as nif cluster; (iii) iron acquisition by siderophore biosynthesis; (iv) phytohormone production; (v) antibacterial and antifungal metabolites including nonribosomal peptides (NRPs), polyketides (PKs), rhamnolipids (RLs) and the metabolite 2,4-diacetylphloroglucinol. Gene clusters associated with the ability of Pseudomonas strains to form biofilm that support root colonization were also seeked. The presence of relevant genes guided in-vitro evaluation of PGPB traits. The production of phytohormones and siderophores was evaluated by colorimetric tests in liquid and on solid media,phosphate solubilization and nitrogen fixation were tested on NBRIP agar plate and in N free medium, respectively. Biofilm formation was assessed in microtiter plate using crystal violet, and biostimulation assays were carried out on tomato seedling. The current study highlights the contribution of genomic mining as a pre-experimental approach in the characterization of plant growth-promoting abilities and biocontrol activity of bacterial strains. This strategy combining bioinoformatics and wetlab experiments seems efficient to target strains potentially displaying relevant traits and allows for time saving in the screening process.
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Dates et versions

hal-04550295 , version 1 (18-04-2024)

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  • HAL Id : hal-04550295 , version 1

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Fatima Ait Koujane, Matthieu Duban, Marlène Chollet, Elodie Dussert, Josette Behra-Miellet, et al.. A combined approach of genome mining and in vitro evaluation of Biocontrol and Plant Growth-Promoting Bacteria (PGPB) functions of Pseudomonas strains. ISCP, May 2023, GHENT, Belgium. ⟨hal-04550295⟩
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